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TB-500 (Thymosin Beta-4 Fragment)

✍️ Reviewed by nnng.com Editorial Team · April 2026 · Medical disclaimer

Systemic tissue repair — accelerates healing of muscle, tendon, heart, and eye tissue.

📍 Healing & Recovery ⏱ 2×/week (loading), 1×/week (maintenance) 💉 SubQ 🔄 Cycle: 4–6 weeks loading, then maintenance ⏳ Half-life: ~3 days ❄️ Storage: 30 days refrigerated

📋 What is TB-500?

TB-500 is a synthetic peptide fragment of Thymosin Beta-4 — a naturally occurring protein found in virtually all human and animal cells. It plays a critical role in cell migration, survival, and differentiation. TB-500 is used for systemic healing and recovery from injury.

Mechanism: TB-500 acts primarily by upregulating actin — the protein that forms the cellular cytoskeleton. By regulating actin polymerization, TB-500 promotes cell migration to injury sites, reduces inflammation, and accelerates tissue repair across multiple tissue types simultaneously.

✅ Key Benefits

Rapid muscle repair and reduced recovery time
Tendon and ligament healing
Cardiac tissue protection and repair
Reduced inflammation and scar tissue formation
Improved flexibility and range of motion
Hair follicle stimulation

🧬 TB-500 Reconstitution Calculator

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⚠️ Research use only. Not medical advice. Consult a licensed healthcare provider before using any peptide.

💉 Dosing Reference Table

Phase	Dose	Frequency	Duration
Loading (acute injury)	2.5 mg	2× per week	4–6 weeks
Loading (general)	2 mg	2× per week	4–6 weeks
Maintenance	1.25–2 mg	1× per week	Ongoing
Low-dose preventive	1 mg	1× per week	Ongoing

Loading phase: 2–2.5 mg SubQ twice per week (e.g., Monday and Thursday) for 4–6 weeks. Then drop to maintenance: 1.25–2 mg once per week. TB-500's longer half-life (~3 days) means twice-weekly dosing provides consistent blood levels throughout the loading phase. Unlike BPC-157, TB-500 is systemic — injection site does not need to be near the injury.

🔀 Common Stacks

+ BPC-157

The gold standard healing stack. BPC-157 + TB-500 addresses tissue repair from multiple pathways simultaneously. Most widely used peptide combination for injury recovery.

+ Ipamorelin + CJC-1295

Adding GH secretagogues amplifies the anabolic and repair signals for more comprehensive recovery support.

❓ Frequently Asked Questions

What's the difference between TB-500 and BPC-157?

TB-500 acts primarily via actin regulation and is more systemic — a single injection affects the whole body. BPC-157 works more locally (especially with IM injection) and focuses on angiogenesis and growth hormone receptor upregulation. They complement each other well in a stack.

Why the loading/maintenance protocol?

TB-500 has a longer half-life (~3 days) than BPC-157 (~4 hours). The loading phase builds up tissue levels and saturates receptors. Once saturated, less frequent maintenance dosing maintains benefits. This mirrors how it's used in veterinary applications (race horses).

Does injection site matter?

For TB-500, no. Because it acts systemically via the bloodstream, SubQ injection anywhere (abdomen is easiest) reaches the target tissues. This is different from BPC-157 where IM near the injury site can provide more concentrated local effects.

Can TB-500 promote cancer growth?

TB-500 upregulates actin, which is involved in cell migration — a concern sometimes raised about potential cancer promotion. Animal studies have not shown oncogenic effects at therapeutic doses. However, individuals with active cancer should avoid TB-500 and consult their oncologist before using any growth-promoting peptide.